Figure 1.

Confirmation of probe β-value methylation by bisulphite sequencing. The regions surrounding a positive probe was amplified and direct sequenced for bisulphite-modified tumor DNA. For each gene, three tumors had 10 clone sequences analyzed and methylation indexes (M.I.s) were calculated for both the target CpG of the relevant Infinium probe or for the total number of CpGs analyzed in the sequence and displayed next to the clone sequencing. Black dots represent methylation present and BstUI digestion sites have been marked on the sequence. The positions of the Infinium probe or probes and the CpG island are mapped to the first exon of the respective genes.