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. 2012 Jan 1;8(1):29–46. doi: 10.4161/auto.8.1.18104

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Copyright © 2012 Landes Bioscience

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FBPase degradation is inhibited in the ΔCTLH mutant. (A) Schematic illustration of the position of the LisH and CTLH domains in Vid30 and the CTLH domain that was deleted to produce the CTLH domain mutant. (B) The CTLH domain of Vid30 was deleted and the mutant protein was tagged with V5-His. FBPase degradation was examined. The levels of ΔCTLH-Vid30-V5-His were determined. (C) Cells that co-expressed ΔCTLH-Vid30-V5-His and Vid24-HA were shifted to glucose for 20 min. Total lysates were prepared and ΔCTLH-Vid30-V5-His was pulled down. The presence of ΔCTLH-Vid30-V5-His, Vid24-HA, Sec28 and FBPase in the unbound and bound fractions was determined. (D) Cells that co-expressed ΔCTLH-Vid30-V5-His and Vid24-HA were glucose starved for three d and re-fed with glucose for 20 min. The distribution of ΔCTLH-Vid30-V5-His, Vid24-HA and FBPase in the Vid vesicle and cytosolic enriched fractions was examined and quantitated using ImageJ software.