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. 2012 Jan 1;8(1):29–46. doi: 10.4161/auto.8.1.18104

Figure 3.

Figure 3

Vid30-GFP is associated with actin patches and FM-containing compartments. (A) Wild-type cells expressing Vid30-GFP were glucose starved and shifted to glucose for 0, 2 and 3 h. FBPase degradation was then examined. (B) The distribution of Vid30-GFP and actin patches was determined. (C) rvs161Δ cells expressing Vid30-GFP were re-fed with glucose and the distribution of Vid30-GFP and actin patches was examined. (D) Wild-type cells expressing Vid30-GFP were transferred to glucose-containing medium in the presence of FM for the indicated periods. The distribution of Vid30-GFP and FM was visualized using fluorescence microscopy.