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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Traffic. 2012 Jan 31;13(4):586–598. doi: 10.1111/j.1600-0854.2011.01326.x

Figure 5. Sna3 is an adaptor protein for the methionine transporter Mup1.

Figure 5

A) Wild-type, sna3Δ and art1Δ cells transformed with a plasmid expressing Mup1-GFP were grown to an optical density OD600 = 1.0 in minimal media. Mup1-GFP internalization was then induced by addition of 20 μg/ml methionine.

B) Cells were harvested at given time points after methionine addition, lysed and subjected to SDS-PAGE and immunoblot analysis using anti-GFP (upper panels) and anti-PGK (lower panels) antibodies.