A) Wild-type, sna3Δ and art1Δ cells transformed with a plasmid expressing Mup1-GFP were grown to an optical density OD600 = 1.0 in minimal media. Mup1-GFP internalization was then induced by addition of 20 μg/ml methionine.
B) Cells were harvested at given time points after methionine addition, lysed and subjected to SDS-PAGE and immunoblot analysis using anti-GFP (upper panels) and anti-PGK (lower panels) antibodies.