Table 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pRS313 | Low copy yeast shuttle plasmid containing HIS3 marker | (53) |
| pRS315 | Low copy yeast shuttle plasmid containing LEU2 marker | (53) |
| pRS316 | Low copy yeast shuttle plasmid containing URA3 marker | (53) |
| Figure 1 | ||
| pPL3667 | pRS316 expressing Sna3-GFP from SNA3 promoter | This study |
| pPL2279 | pRS316 expressing Sna3ΔK-GFP from SNA3 promoter. Sna3 (K19R K125R) | This study |
| pPL4618 | pRS316 expressing Sna3-2xHA from SNA3 promoter | This study |
| pPL4619 | pRS316 expressing Sna3ΔK-2xHA from SNA3 promoter. Sna3 (K19R K125R) | This study |
| Figure 2 | ||
| pPL2089 | pRS315 expressing Sna3-GFP from SNA3 promoter | This study |
| pPL3886 | pRS316 expressing Sna3-GFP-Ubp7 from SNA3 promoter. Ubp7 residues 561–1071 | This study |
| pPL3904 | pRS316 expressing Sna3-GFP-ubp7* from SNA3 promoter. pPL3904 with Upb7 C618S mutation | This study |
| pPL3874 | pRS313 expressing Hse1-Ubp7 from CUP1 promoter. Ubp7 residues 561–1071 | This study |
| pPL4141 | pRS315 expressing Hse1-ul36* from CUP1 promoter. Inactive UL36 catalytic domain | (32) |
| pPL3742 | pRS316 expressing Rsp5-Ubp7-3xHA from CUP1 promoter. Active Ubp7 catalytic domain | (32) |
| pPL4502 | pRS316 expressing Rsp5-ubp7*-3xHA from CUP1 promoter. Inactive Ubp7 catalytic domain | (32) |
| Figure 3 | ||
| pPL4708 | pRS315 expressing Sna3-GFP (pPL2089) from SNA3 promoter converted to ADE2 | This study |
| pPL4772 | pPL4708 expressing HA-Rsp5C777A from RSP5 promoter. | This study |
| pRS416-RSP5 | pRS416 expressing Rsp5 from RSP5 promoter. | (54) |
| Figure 5/6 | ||
| pCHL642 | pRS416 expressing Mup1-GFP from MUP1 promoter | (11) |
| pPL4621 | pRS316 expressing Sna3ΔPY-2xHA from SNA3 promoter. Sna3 P106PAY mutated to G106GAA | This study |
| Figure 7 | ||
| pPL4146 | pRS315 expressing Mup1-GFP from CUP1 promoter | (32) |
| pPL4064 | pRS316 expressing Sna3-mCherry from SNA3 promoter | This study |
| pPL4066 | pRS316 expressing Sna3-mCherry-Ubp7 from SNA3 promoter. Ubp7 residues 561–1071 | This study |
| pPL4566 | pRS316 expressing Sna3ΔPY-GFP from SNA3 promoter. P106PAY mutated to G106GAA | This study |
| pPL4568 | pRS316 expressing Mup1-mCherry-Ub from CUP1 promoter | This study |