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. 2012 Feb 1;13(3):130–137. doi: 10.4161/cbt.13.3.18690

Figure 4.

Figure 4

Effect on signaling after AG1478 and calcium treatment. (A) In vivo: mice maintained on a control or calcium supplemented diet, with or without AG1478, were injected with phosphatase inhibitor prior to euthanasia. EGFR activation was measured in liver protein lysates from ApcMin/+ and AOM mice, separately for each strain and sex. (B) In vitro: protein was extracted from human CRC cells lines after a 4 h treatment with or without 4 µM (Caco-2) or 8 µM AG1478 (HCT116) with 3.5 mM (“+”) and 4.5 mM (“++”) calcium chloride. (C) pEGFR ELISA was performed using protein extracted from cells lines after 2 h treatment with or without 4 µM (Caco-2) or 8 µM AG1478 (HCT116) with 3.5 mM (“+”) and 4.5 mM (“++”) calcium chloride followed by 20 min treatment with EGF.