Figure 3.

CCKCR phenotype is associated with the presence of the SNP A/A or A/C genotype. (A) CCKCR immunoreactivity is found in malignant cells of a pancreatic cancer of a patient having genotype A/A. CCKCR immunoreactivity is identified in a nerve invaded by cancer cells (arrow). The surrounding connective tissue (*CT) is void of CCKCR staining. (B) CCKCR positive immunoreactivity is seen from a pancreatic tumor of a patient with genotype A/C (arrows point to cancer cells). Again note the fibrous connective tissue surrounding the cancer cells is void of receptors. (C) A pancreatic cancer specimen from a subject with genotype C/C shows lack of CCKCR immunoreactivity. Arrow is pointing to malignant glands where cancer cells do not have the CCKCR. (D) A tissue specimen from a procured normal pancreas with genotype A/A showing the lack of CCKCR immunoreactivity in normal benign tissue. (E) PANC-1 cancer cells which have the A/C genotype show immunoreactivity to the CCKCR antibody by confocal microscopy. (F) AsPC-1 pancreatic cancer cells which have the C/C genotype do not react with the CCKCR antibody. Nuclei are stained with DAPI.