Figure 3.

Chemotherapeutic drugs induce autophagy in the 67NR and 4T1 cell lines. Cells were treated with 1 mM cisplatin, (Cisp) for 6 h or with 30 µM LY294002 (LY) or 0.2 µM rapamycin (Rapa) for 8 h ± CQ and proteins were collected for WB (A). GFP-cherry-LC3 expressing 67NR cells grown in coverslips were treated with the same drug concentrations for 8 h, fixed and observed in a confocal microscope. The bar in merged images represents 20 µM and the one in the inset represents 10 µM. Quantification of yellow dots is shown in (C). Graph shows mean ± standard error of three independent experiments.