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. 2012 Feb 1;8(2):236–251. doi: 10.4161/auto.8.2.18600

Figure 6.

Figure 6

Expression of p18-CycE is regulated by autophagy. (A) Total protein lysates were immunoblotted for HA-p18-CycE and β-actin at the indicated time following IR. (B) Cells were lysed at the indicated time following IR and/or chloroquine (100 µM) treatment and immunoblotted for HA-p18-CycE using anti-HA antibody and β-actin as loading control. (C) Confocal co-immunostaining for HA-p18-CycE in parental and p18-CycE-expressing cells at 24 h following IR with or without chloroquine. (D and E) Confocal co-immunostaining for HA-p18-CycE/LC3I/II and HA-p18-CycE/LAMP2 in cells stably-expressing p18-CycE at the indicated time following IR. (F and G) Confocal co-immunostaining for HA-p18-CycE/LAMP2 in p18-CycE-expressing cells with ATG7 or LAMP2 knockdown at the indicated time following IR. Nuclei were stained with DAPI.