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. 2012 Feb 1;8(2):236–251. doi: 10.4161/auto.8.2.18600

Figure 7.

Figure 7

DNA damage induces autophagy-dependent senescence in p18-CycE-expressing cells. (A) SA-β-Gal-positive, p18-CycE in the absence or presence of shATG7-expressing cells at 6 d post IR. ***p = 0.003, **p = 0.005, *p < 0.001 Student's t-test. Data represent means ± s.e.m. of three independent experiments done in triplicates. (B) Expression of HP1γ and β-actin in parental and p18-CycE expressing cells in the absence or presence of shATG7 at 6 d post IR. Fold change in HP1γ is indicated. (C and D) Visualization of endogenous HP1γ and γH2AX-associated foci by confocal immunostaining in parental and p18-CycE-expressing cells in the absence or presence of shATG7 expression following IR. (E and F) Percentage of cells having at least 5 foci per cell for γH2AX and HP1γ in parental and p18-CycE-expressing cells in the absence or presence of shATG7 expression following IR.