Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Jan 12;32(3):623–632. doi: 10.1161/ATVBAHA.111.242180

Figure 1. Development of a thirteen-parameter immunophenotyping study of leukocytes in the murine carotid artery.

Figure 1

A, Disturbed flow was induced in the LCA of ApoE−/− mice by partial ligation surgery, while the contralateral RCA was used as an internal control. B, Ligated mice were fed a HFD for 4 to 28 days. Shown is a representative micrograph displaying diffuse atherosclerosis (yellow arrows) in LCA, but not in RCA, two weeks post-ligation. C, LCAs (dashed red box) and RCAs (dashed blue box in (B) obtained from 3 mice at a given time point were pooled (regarded as n=1) and vascular leukocytes extracted. D, Shows the gating strategy used for flow cytometry analyses for a representative arterial leukocyte sample harvested from LCA 7 days post-ligation. Ultra-bright counting beads (D2) were used to calculate absolute cell counts. Gate numbers indicate percent of parent. FSC-H, forward scatter height; FSC-A, forward scatter area; SSC-A, side scatter area.