Figure 4.

Measurements of Oxalate Degradation to CO₂.

(A) Schematic of proposed oxalate degradation pathway in Arabidopsis.

(B) Radiolabeled CO₂ measurements. Leaf discs of the wild type (WT), aae3-1, and aae3-2 were fed with 5 μCi of [¹⁴C] oxalate along with 500 μM nonlabeled oxalate for 5 h. The CO₂ evolved was captured using 1 M KOH and the radioactivity measured by scintillation counting. Data are mean ± se (n = 4). Asterisks denote significant difference from the wild type (*P < 0.05; Student’s t test).