Figure 7.

Oxalate Measurements in Rosette Leaves.

(A) to (C) Oxalate contents were determined by HPLC for leaves of the wild type (WT), aae3-1, and aae3-2. The total (A), soluble (B), and insoluble (C) oxalate contents are shown. Soluble oxalate was extracted with water from 20 mg lyophilized leaf tissue, while the remaining insoluble fraction was solubilized by incubation at pH 2.8 and 65°C for 1 h. The data are mean ± se (n = 8).

(D) Oxalyl-CoA synthetase activities in crude extracts from wild-type leaves and mature siliques measured using 300 μM oxalate as substrate. The data are mean ± se (n = 3).

Asterisks denote significant difference from the wild type (*P < 0.05; Student’s t test).