Figure 2.

The PHOT2 Kinase Domain Interacts with 14-3-3 λ.

The β-Gal activity (LacZ reporter gene) was used to determine the strength of interaction between different domains of PHOT2 and 14-3-3 λ in liquid assays using o-nitrophenyl-β-d-galactopyranoside as a substrate. The PHOT2 kinase domain alone interacts as strongly as the full-length PHOT2. Little or no β-Gal activity was detected when the putative 14-3-3 λ recognition site that includes Ser-747 of the PHOT2 kinase domain was replaced with Ala. Representative data (mean ± sd; n = 3 yeast colonies) from two different experiments with similar results are shown. Numbers labeled on the representation of PHOT2 start from the initiation Met (1).