Figure 8.
Increased DNA Methylation in F1 Hybrids Altered the Expression of DNA Methylation-Sensitive Genes.
(A) Number of genes that were shown to be upregulated in ddc mutants (Kurihara et al., 2008) but were downregulated (top panel) or upregulated (bottom panel) in 15-d-old F1 hybrids of Ler and C24.
(B) Real-time qRT-PCR analysis showing downregulation of CCA1 and LHY and upregulation of TOC1 and GI expression in F1 hybrids of Ler and C24. Seedlings grown 15 d under white light conditions (16 h light/8 h dark) were collected and subjected to real-time qRT-PCR analysis. Error bars represent sd of triplicate experiments.
(C) Real-time qRT-PCR analysis showing downregulation of three genes (CHS, F3H, and FLS) involved in flavonoid biosynthesis in 15-d-old F1 hybrids of Ler and C24. Error bars represent sd of triplicate experiments.
(D) Auxin transport in Ler and C24 parental lines and their reciprocal hybrids tested by 3H-IAA assays. Error bars represent sd (n = 4). *P < 0.05 (Student’s t test) for the indicated pair of samples. as, apical side; bs, basal side.
(E) Real-time qRT-PCR analysis showing upregulation of three auxin-related genes (AVP1, NAC1, and ARGOS) in 15-d-old F1 hybrids of Ler and C24. Error bars represent sd of triplicate experiments.