Figure 5.

Phased Secondary siRNAs Initiated by miR482 Target Sites in an NBS-LRR mRNA.

Genome view of a CNL-type NBS-LRR locus (LRR1) that aligns with phased secondary siRNAs. Most of the secondary siRNAs align between miR482 target sites. Red bars indicate the start position of sRNAs in phase; blue bars indicate the start position for those out of phase. Rectangles indicate expected phased positions and those in red indicate phased sequences. Orange box indicates position of P-loop. Numbers next to the miR482 target site indicate frequency of 5′ RACE clones matching this prediction out of total clones used for analysis. Arrow color code: red, 21 nucleotides; blue, 24 nucleotides; green, 22 nucleotides. The black triangle indicates cleaved site, and the white triangle indicates proposed cleavage site.