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. 2012 Mar 16;24(3):1066–1080. doi: 10.1105/tpc.111.095059

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 7. — CSI1 Binds to Microtubules in Vitro.

Equal amounts of His-Tagged CSI1 were used in pull-down experiments in the presence (+) or absence (−) of microtubules that had been stabilized by taxol treatment. CSI1 protein was detected in the pellet [P] rather than the supernatant [S] after centrifugation. A control protein (At5g16050) showed no preferential copurification with microtubules. An isolated microtubule-associated protein fraction from bovine brain (MAPF) was used as positive control, and BSA was used as negative control. Molecular weight of bands: His-tagged CSI1, ~230 kD; MAPF (MAP1 and MAP2), ~250 kD; BSA, 68 kD.