Table 1.
Measurements on a sample of five different fluorescent beads without out-of-focus light. These are the measured mean widefield intensity 〈f〉 and its standard deviation σm(f), the calculated widefield intensity 〈iw〉 and its standard deviation σm(iw), and the corresponding ratios r obtained with Eq. (17). The modulation contrast m used to calculate 〈f〉 is estimated using Eq. (15).
| Sectioned image
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Calculated widefield image
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| Bead | 〈f〉 (cts) | σm(f) (cts) | r | 〈iw〉 (cts) | σm(iw) (cts) | r |
| 1 | 5.03 × 104 | 279 | 2.53 | 4.99 × 104 | 114 | 1.03 |
| 2 | 6.29 × 104 | 292 | 2.35 | 5.51 × 104 | 122 | 1.06 |
| 3 | 4.61 × 104 | 259 | 2.44 | 4.59 × 104 | 115 | 1.08 |
| 4 | 5.04 × 104 | 277 | 2.50 | 4.93 × 104 | 110 | 1.00 |
| 5 | 6.17 × 104 | 276 | 2.24 | 5.60 × 104 | 119 | 1.02 |
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| mean: | 2.41 | mean: | 1.04 | |||