Fig. 2.

Growth analysis of CAP-H and CAP-D3 knockout cell lines. (A) Growth curves of parental DT40 cells and CAP-H and CAP-D3 knockout (KO) cell lines in the presence or absence of doxycycline (dox). (B) Phase-contrast images of SMC2, CAP-H and CAP-D3 KO cell lines in the absence or presence of doxycycline for up to 96 hours. (C) Scoring of polyploid cells for condensin KOs. PFA-fixed, DAPI-stained cells counterstained with antibodies against lamin B1 or β-tubulin were used to quantify the amount of binucleate or giant cells (grouped together as polyploid cells) and expressed as a percentage of the total population. Examples of DAPI images of normal-sized, binucleate and giant cells are presented above. Error bars represent the standard error.