Fig. 7.

Intrinsic metaphase structure (IMS) assay. Chromosome morphology of TEEN-treated CAP-H and CAP-D3 KO cells were grouped into the following categories, ranging from less to more disorganized: ‘retain chromosome morphology’, ‘lost lateral compaction’ and ‘totally disorganized’. Representative images from each category are shown, the data were tabulated below and scored from the following numbers of metaphases: CAP-H 0 hours doxycycline (dox; N=16), CAP-H +dox 24 hours (N=15), CAP-H +dox 48 hours (N=14), CAP-D3 0 hours dox (N=17), CAP-D3 +dox 24 hours (N=27), CAP-D3 +dox 48 hours (N=24). Chromosomes were stained with DAPI (white). All images are at the same scale. Scale bar: 5 μm.