Figure 11.

Synaptic protein levels are altered in APP-transgenic mice. Brain homogenates from APdE9 double-transgenic mice, APdE9/RanBP9 triple-transgenic mice, and age-matched WT controls prepared using Nonidet P-40 lysis buffer were subjected to SDS-PAGE electrophoresis and probed with antibodies against postsynaptic protein, PSD-95 (A, B) and presynaptic protein, synaptophysin (C, D). Same blot was reprobed to detect flag-tagged RanBP9 and endogenous RanBP9. ImageJ quantification showed decreased levels of PSD-95 (B) by 55% in APdE9 mice compared to WT mice, and the levels were further decreased in APdE9/RanBP9 mice to ∼71%; synaptophysin levels (D) were decreased by 25% in APdE9 mice and by 32% in APdE9/RanBP9 triple-transgenic mice. Data are presented as means ± se (n=4/group for WT; n=5/group for APdE9 and APdE9/RanBP9). ***P < 0.001 vs. WT, ^$P < 0.05 vs. APdE9/RanBP9; ANOVA followed by post hoc Tukey's test.