Figure 5.

RanBP9 protein is localized throughout the neuron, including neurites. Primary hippocampal neurons from P1 pups were cultured for 18–20 d in vitro to allow for the formation of complex network of neurites and then stained with RanBP9 antibody. A) Negative control without the primary antibody revealed no immunoreactivity, indicating that the immunofluorescence signal observed in B is specific to RanBP9. B) Two neurons showing robust expression of RanBP9 protein throughout the neuron, though to a lesser extent at the center of the neuron. C) Complex network of neurites showing punctate staining for RanBP9. D, E) staining of neurons in an adult mouse brain cortex (D) and hippocampus (E), showing the presence of RanBP9 protein throughout the neuron, including processes.