Figure 7.

RanBP9 overexpression decreases postsynaptic protein at 12 mo of age. A) Brain homogenates from RanBP9-transgenic (TG) and age-matched WT controls prepared using Nonidet P-40 lysis buffer were subjected to SDS-PAGE electrophoresis and probed with antibodies against postsynaptic proteins PSD-95, spinophilin, and drebrin A. Same blot used in Fig. 9 for presynaptic proteins is also shown here for the detection of flag-tagged RanBP9 and endogenous RanBP9. B) ImageJ quantification showed decreased levels of PSD-95 by 19%, spinophilin by 7%, and drebrin A by 10%. Data are presented as means ± se (n=4/group). **P < 0.01 vs. WT; Student's t test.