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RanBP9-null mice show complete absence of RanBP9 protein. A) Nonidet P-40 lysates from WT, RanBP9 heterozygous (+/−), and RanBP9 homozygous (−/−) mice subjected to immunoblotting revealed presence of endogenous RanBP9 protein in WT, reduced levels in +/−, and complete absence of RanBP9 protein in −/− mice. *NS, nonspecific band. B) Immunohistochemical staining of cortical brain sections from RanBP9−/− mice with X-gal revealed expression of RanBP9-β-gal fusion protein.
