Figure 5.

Differential effect of hypoxia on AKT and 4E-BP1 phosphorylation in JEG-3 and BeWo cells. JEG-3 cells and BeWo cells were incubated either at 20 or 1% O₂ for 3 d. A) Proteins were isolated and subjected to Western blot analysis to measure P-AKT (Thr308), P-AKT (Ser473), AKT, P-4E-BP1 (Ser65) and 4E-BP1. β-Actin was used as loading control. B) Densitometric quantification of band intensity. Levels of phosphorylated and total protein are presented as a ratio to the untreated control (100%). Data are means ± sd from 3 independent experiments. *P < 0.05; **P < 0.01.