Figure 2.

L. reuteri altered enterocyte RNA pathways involving cell motility. A, B) IPA of differentially expressed enterocyte genes showing the 5 most significantly altered canonical pathways and the highest-scoring protein interaction network for each probiotic strain vs. PBS (n=3 mice/group), 24 h after gavage with strain 17938 (A) or strain 6475 (B). C) Immunohistochemistry reveals diminished production of stabilizing cytoskeletal protein β-actin (product of ACTB gene in above networks) in enterocytes. Compared to PBS controls (left panel), decreased labeling at the basement membrane was found in mice treated with L. reuteri strains 17938 (center panel) or 6475 (right panel). However, smooth muscle cell β-actin (arrowheads) was labeled in all treatment groups. Red represents up-regulated genes; green represents down-regulated genes; gray represents genes not differentially expressed; white represents genes with no array data; solid lines/arrows represent direct interactions; dashed lines/arrows represent indirect interactions.