Figure 3.

Expression of BMP7 ligand and receptors in the hypothalamus. A) In situ hybridization analysis of hypothalamic sections for BMPRII (pink), showing caudal hypothalamus (left panel) and rostral hypothalamus (right panel). Antisense represents specific staining (top panel), while sense staining represents background (bottom panel). BMPRII, a type 2 BMP receptor, was highly expressed throughout the hypothalamus. B, C) Immunofluorescence staining (green FITC signal) for the type 1 BMP receptors ALK3 (B) and ALK2 (C) in the mouse hypothalamus, along the third ventricle. ALK3 staining was most robust in the arcuate and paraventricular nuclei, while ALK2 staining was most robust in the dorsomedial and paraventricular nuclei. D) LacZ staining of mouse hypothalamus (top panel) and dorsal midbrain (including ventricular system and choroid plexus; bottom panel) showing BMP7-positive cells (blue) in BMP7 reporter mice. Arrowheads indicate choroid plexus surrounding the ventricles (bottom panel).