Fig. 6.

PS_AE (eq. 12) as a function of simulated cellular PAN concentration (eq. 10). A, rAbcg2. B, human ABCG2-MDCKII cell monolayer. Squares indicate (+)PAN; circles, (−)PAN; open symbols, basolateral-to-apical PS_AE; and closed symbols, apical-to-basolateral PS_AE. The initial estimates of PS_AE (eqs. 8 and 9) were used to generate cellular concentration (eq. 10), and these concentrations were used in the final estimation process. Both axes were log-transformed for easier visualization; fitting was performed using untransformed pooled data from both directional PS_AE. The solid and dashed lines are the fitted results for the (−)PAN and (+)PAN isomers, respectively.