TABLE 2.
Effects of human D1 receptor TM5 serine mutations on DA-stimulated cAMP production
Dopamine dose-response curves were obtained in the presence of 500 μM IBMX. Experiments were performed in 48-well plates and cAMP levels were calculated for each well (total volume of 100 μl). Data represent means ± S.E.M. as calculated from at least six independent experiments.
| Cell Line | Basal cAMP | Dopamine |
|
|---|---|---|---|
| EC50 | Emax | ||
| pmol/well | nM | pmol/well | |
| HEK hD1 WT | 3.7 ± 0.7 | 22 ± 3.4 | 134 ± 10 |
| HEK hD1 S198A | 1.8 ± 0.6 | 7800 ± 570** | 117 ± 10 |
| HEK hD1 S199A | 1.3 ± 0.3* | 2800 ± 400** | 111 ± 5.9 |
| HEK hD1 S202A | 1.4 ± 0.4 | 12000 ± 370** | 55 ± 3.1** |
**
Significantly different from wild-type (p < 0.01, one-way ANOVA with Dunnett's post-test).