Fig. 1.

Concentration-response curves for glycine-activated chloride currents in X. laevis oocytes expressing WT and mutant α1GlyR subunits. Glycine-induced chloride currents were normalized to the maximal current activated by a saturating concentration of glycine (30 mM). The curves represent nonlinear regression analysis of the glycine concentration responses in the α1 mutant GlyRs compared with WT α1GlyRs. Details of EC₅₀, Hill slope, and maximal current amplitude (I_max) are provided in Table 1. Glycine was applied for 30 s. Washout time was 5 to 15 min after the application of glycine. Each data point represents the mean ± S.E.M. from eight different oocytes.