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. 2012 Jul;23(2):69–77. doi: 10.7171/jbt.12-2302-003

FIGURE 4.

FIGURE 4

The second round of the Three-step MACS doubles the purity of the first round. HEK293 cells expressing the BAP-Lngfr-EGFP fusion protein on their cell surface were added into dissociated, 12.5 d.p.c. mouse embryo cells. In the first round, they were sorted with anti-biotin; in the second round, with anti-Lngfr. The purity of the second and third round of sorting was 37.9% (B) and 84.4% (C), respectively. The first panel is a FC plot of the baseline control using dissociated mouse embryo cells without any BAP-Lngfr-EGFP-transfected HEK293 cells spiked in.