Fig. 4.

MS³ spectra and fragmentation assignments of the m/z 1476 B-ion from GlcNAc₂Man₅Fuc (896²⁺) in N2 and NF299. Fragmentation profiles differ between the wild-type and COG-deficient strain illustrating changes in fucosylation. The wild-type N2 strain added a terminal fucose residue to the antenna and is evident from the m/z 1288, 1231, 707 and 433 ions. Fucose position in the NF299 strain was at the non-reducing core GlcNAc with an additional terminal hexose. This motif was apparent from the m/z 941, 871 and 646 ions as well as the absence of m/z 1288 ion, corresponding to the neutral loss of a terminal fucose.