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Quantification of PPi levels in conditioned medium corrected for DNA content, and visualization of the mineralization by Alizarin red staining, in differentiated Vdr+/+ osteoblasts after treatment for 4 days with vehicle (veh), 10–8 M 1,25(OH)2D3 (1,25D), 1 U/ml TNAP, and 5 × 10–6 M PPi as indicated. n = 6. *P < 0.05 as indicated by brackets, 1-way ANOVA followed by Fisher’s LSD multiple-comparison test.
