Figure 3. CD4⁺ or CD8⁺ T cell vaccination to A. fumigatus requires distinct TLR signaling.

Dectin-1, TLR-, MyD88-, or TRIF-deficient mice were administered A. fumigatus conidia or the protective recombinant fungal antigens (Pep1p, Gel1p, or Crf1p) plus CpG and assessed for resistance to reinfection in terms of (A) survival (%), (B) fungal growth (log₁₀ CFU ± SEM), and (C) proliferation of CD4⁺ or CD8⁺ T cells, purified from Pep1p- or conidia-vaccinated mice, respectively, at 3 days after infection, and cultured in vitro with conidia- or Pep1p-pulsed DCs from the corresponding naive mice. DNA synthesis was measured by ³H-thymidine uptake after 72 hours coculture. *P < 0.05, **P < 0.01, ***P < 0.001, vaccinated versus unvaccinated (None) mice. ND, not done. Data are representative of 3 experiments.