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. 2012 Apr 23;122(5):1816–1831. doi: 10.1172/JCI60862

Figure 4. CD4+ and CD8+ T cells are activated through distinct intracellular antigen presentation pathways.

Figure 4

CD8+ or CD4+ T cells were purified from lungs of C57BL/6 or p47phox–/– mice a week after the intranasal infection and exposed to conidia-pulsed (A) or Pep1p-pulsed (B) DCs purified from lungs of the corresponding naive mice. Prior to the 2-hour pulsing with conidia or Pep1p, DCs were exposed to the indicated antigen presentation pathway inhibitors for 120 minutes. (C) CD8+ T cells, purified as in A, were exposed to DCs and/or intact or apoptotic (Apo) alveolar macrophages (AM), purified from lungs of the corresponding naive mice. AMs were pulsed to live conidia before the induction of apoptosis with LPS plus ATP. Cells were assessed for proliferation and Ifng expression by RT-PCR, 72 hours after the coculture. DNA synthesis was measured by 3H-thymidine uptake. Data are from 4 independent experiments.*P < 0.05, **P < 0.01, inhibitors versus no inhibitors; and DC-exposed versus T cells alone (Ct).