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. 2012 Apr 16;122(5):1644–1652. doi: 10.1172/JCI61429

Figure 2. Infectivity of WT and Gag30 mutant HIV-1 and SIVcpz strains in TZM-bl cells.

Figure 2

(A) TZM-bl indicator cells were infected with WT and Gag30 mutant SIVcpz and HIV-1 constructs as indicated. Infections were performed in triplicate with virus stocks containing 1 ng of p24 antigen (average values ± SDs are shown). CPZa, chimpanzee adapted. (B) Relative infectivity of WT and Gag30 mutant HIV-1 and SIVcpz constructs. Shown are minimum and maximum values, 25% and 75% percentiles, and median values. (C) Modulation of viral infectivity by host-specific adaptation at Gag30. Changing a Lys or Arg to a Met or Leu reduced the infectivity of HIV-1 by 50%, while the reciprocal mutations more than doubled the infectivity of SIVcpz. Values are shown in relation to those of the corresponding parental HIV-1 or SIVcpz constructs set to 100%.