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. 2012 Apr 16;122(5):1644–1652. doi: 10.1172/JCI61429

Figure 4. Replication kinetics of WT and Gag30 mutant HIV-1 and SIVcpz in human tonsil explant cultures.

Figure 4

(A) Representative growth curves of WT and Gag30 mutant HIV-1, CPZ-adapted HIV-1 (CPZa), and SIVcpz constructs. Infections were performed in triplicate. Data shown are average values ± SD. (B) Virus production by tonsillary explant cultures infected with SIVcpz and HIV-1 constructs. Cumulative virus production is shown over 14 days. Matched tissues from 7 to 12 donors were inoculated with the WT HIV-1 (left), chimpanzee-adapted HIV-1 (middle). and SIVcpz (right) constructs or with their respective Gag30 mutants, and for each HLT culture, virus production was measured over 14 days. Data are shown as mean ± SEM. (C) Average cumulative p24 antigen levels in HLT cultures from 7 to 12 donors. Data are shown as mean ± SEM. (D) Host-specific adaptation increases replication fitness. Mutation of K/R30M/L reduces HIV-1 replication, and substitution of M/L30R increases SIVcpz replication in HLTs. Cumulative p24 antigen production levels of the Gag30 mutants are shown in relation to those of their respective parental constructs set to 100%.