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. 2012 Apr 16;122(5):1869–1880. doi: 10.1172/JCI61492

Figure 5. ERK3 and SRC-3 cooperatively regulate MMP gene expression.

Figure 5

(A–C) Depletion of SRC-3 or ERK3 significantly decreased (A) MMP2, (B) MMP9, and (C) MMP10 gene expression. MMP gene expression in H1299 cell pools stably expressing shSRC-3, shERK3, or shCtrl was determined by RT-qPCR analysis. *P < 0.01, **P < 0.005 (Student’s t test). (D) SRC-3 and ERK3 cooperatively promote MMP2 promoter-driven luciferase activity, in which S857 of SRC-3 plays an important role. A549 cells were singly transfected or cotransfected with the constructs as indicated. Luciferase activity is represented as relative luciferase units (RLUs) on the y axis. *P < 0.01 (Student’s t test). (E) Knockdown of ERK3 significantly decreased SRC-3–induced MMP2 promoter-driven luciferase activity. A549 cells were transfected with SRC-3Flag or the empty vector or together with either ERK3 siRNA or nontargeting control siRNA (Ctrl siRNA). *P < 0.05, **P < 0.001 (Student’s t test). (F) Knockdown of SRC-3 significantly decreased ERK3-induced MMP2 promoter-driven luciferase activity. A549 cells were transfected with Myc-ERK3 or the empty vector or together with either SRC-3 siRNA (siSRC-3) or nontargeting control siRNA (siCtrl). Values represent the mean ± SEM of 3 separate experiments. *P < 0.01 (Student’s t test).