Fig. 4.

Alpha-synuclein protein over-expression in brain regions of Thy1-aSyn mice assessed by Western blotting using an antibody against mouse and human alpha-synuclein (mouse anti-alpha-synuclein); clone 42, 1:3000 (BD Biosciences, San Jose, CA). Left: Western blot of alpha-synuclein in the substantia nigra. Right: Alpha-synuclein expression (17 kDa) in fold change compared to wild-type (wt). Thy1-aSyn (tg) mice brain in comparison to wt. Normalized to alpha-tubulin for loading control. Substantia nigra (SN), striatum (str), thalamus (thal), cortex (cor), frontal cortex (f.cor), hippocampus (hipp), cerebellum (CB), and olfactory bulb (OB) (mean ± SEM of 3-6 mice; *p < 0.05; **p < 0.01; ***p < 0.001; 2-way repeated measures (RM) analysis of variance, main genotype effect p < 0.001, student’s t-test planned comparison for each subregion