Table 2.
Power Analysis for Major Endpoints in Thy1-aSyn Mice
| Tests | Parameters with Impairment or Changes in Thy1-aSyn Mice (Major Endpoints)-Age | n for 80% Power to Detect % Improvement |
|---|---|---|
| Beam motor test | Errors per step (increased) 4 months | n = 16 for 30% |
| Pole motor test | Time to turn, time to descend (increased) 4 months | n = 16 for 30% |
| Open field test | Total move time (increased in young mice) 7 months | n = 2 for 30% |
| Olfaction skills | Time to find buried food reward (increased) 4.5 months | n = 16 for 40% |
| Cognitive function | Object place recognition (decreased) 4.5 months | n = 9 for 50% |
| Cognitive function | Alternations in Y-maze (decreased) 7-9 months | n = 7 for 50% |
| Adhesive removal | Time to remove adhesive sticker (increased) 14 months | n = 18 for 30% |
| Catalepsy test | Latency to move (increased) 14 months | n = 20 for 50% |
| Substantia nigra | Alpha-synuclein positive aggregates (transgenics) 5 months | n = 6 for 30% |
| Inflammation | Microglia (IBA-1) activation (increased) 5 months | n = 6 for 12% |
| Striatum HPLC | Dopamine and metabolites (decreased) 14 months | n = 18 for 50% |
“Power” is defined as the probability of correctly rejecting the null hypothesis. The Student’s t test sample size is determined using the following parameter: expected difference in means, expected standard deviation (drawn from previous experience with data), desired power, and alpha (probability to incorrectly rejecting the null hypothesis, set to 0.05). Sufficiency is considered to be 80% power. HPLC = High-performance liquid chromatography