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. 2012 Apr 4;32(14):4724–4742. doi: 10.1523/JNEUROSCI.5602-11.2012

Figure 6.

Figure 6.

Cerebellar pinceau disorganization and infiltration of glial processes in Nfasc-deficient Purkinje and basket neuron cerebella. A–C, Electron micrographs of the pinceau region of cerebellar sections from one-month-old wild-type (+/+) pinceau show compact, electron-dense basket axon collaterals (B) surrounding the Purkinje soma/AIS highlighted by red line (A). Note the presence of synapses between Purkinje (P) AIS and basket collaterals (B) (A, arrowheads). In 1-month-old Pcp2-Cre;NfascFlox cerebella, the pinceau appears slightly disorganized, with spaces beginning to form between basket axon terminals (B) (B, arrows). Note the presence of a synapse between the basket terminal and the Purkinje soma (B, arrowhead). In 1-month-old Parv-Cre;NfascFlox cerebella, the pinceau appears more disorganized with glial processes infiltrating spaces between basket axon terminals (C, arrows). D–F, Electron micrographs at higher magnification showing synapses (black arrowheads) with presynaptic (basket terminal) and postsynaptic (Purkinje AIS) areas in the wild-type (D), Pcp2-Cre;NfascFlox (E), and Parv-Cre;NfascFlox (F) cerebella. G–I, Electron micrographs from cerebellar sections from 3-month-old wild-type cerebella show highly compact basket terminals surrounding the Purkinje AIS (G). Pcp2-Cre;NfascFlox pinceau at 3 months shows increased infiltration of glial processes (H, black arrows). A 3-month-old Parv-Cre;NfascFlox pinceau shows increased disorganization of basket terminals surrounding the Purkinje soma/AIS (I, black arrows). J–O, Electron micrographs of the pinceau from 4-month-old wild-type (J, M), Pcp2-Cre;NfascFlox (K, N), and Parv-Cre;NfascFlox (L, O) cerebella. Wild-type pinceau show compact basket axon terminals surrounding the Purkinje soma/AIS. The Pcp2-Cre;NfascFlox and Parv-Cre;NfascFlox cerebella show severely disorganized basket terminals with increased infiltrating glial processes (black arrows). In some instances, in Parv-Cre;NfascFlox cerebella the entire pinceau areas are completely disrupted (O, black arrows). Scale bars: 0.5 μm.