Purkinje neuron-specific ablation of Nfasc abolishes Purkinje neuron spontaneous activity and reduces inhibitory inputs to Purkinje neurons. A, Representative traces of spontaneous Purkinje neuron firing in wild-type (+/+) and Pcp2-Cre;NfascFlox Purkinje neurons. B, Plot of the spontaneous firing rates of wild-type (black circles) and Pcp2-Cre;NfascFlox mutant (white circles) Purkinje neurons. Larger diamonds show average spontaneous firing rates of Purkinje neurons in wild-type (black diamond) and Pcp2-Cre;NfascFlox mutants (white diamond). C, Representative traces of the action potential train response of Purkinje neurons to injected current at increasing depolarizing steps. D–F, Output responses from wild-type (black circles) and Pcp2-Cre;NfascFlox (white circles) Purkinje neurons to increasing levels of injected current. G, Representative traces of mIPSC recordings from wild-type and Pcp2-Cre;NfascFlox Purkinje neurons. H, Plot of mIPSC frequency reveals decreased frequency in Pcp2-Cre;NfascFlox mutant Purkinje neurons. I, Plot of mIPSC amplitude revealing no change between the wild-type and Pcp2-Cre;NfascFlox mutant Purkinje neurons.