Table1.
Primers of osteogenic genes for qPCR analysis
| Gene name | GeneBank accession no. | 5′–3′ | Probe name |
|---|---|---|---|
| actin-F | NM_007393.3 | aaggccaaccgtgaaaagat | #64 |
| actin-R | gtggtacgaccagaggcatac | ||
| Sp7-F | NM_130458.3 | ctcctgcaggcagtcctc | #106 |
| SP7-R | gggaagggtgggtagtcatt | ||
| OPN-F | AF515708.1 | cccggtgaaaggactgatt | #82 |
| OPN-R | ttcttcagaggacacagcattc | ||
| Osteocalcin-F | NM_001032298.2 | agactccggcgctacctt | #32 |
| Osteocalcin-R | ctcgtcacaagcagggttaag | ||
| Runx2-F | NM_001146038.1 | gcccaggcgtatttcaga | #34 |
| Runx2-R | tgcctggctcttcttactgag | ||
| ALP-F | NM_007431.2 | cggatcctgaccaaaaacc | #12 |
| ALP-R | tcatgatgtccgtggtcaat |