Figure 2. Transposition reporter construct. The reporter construct contains an egfp open reading frame with an insertion of a random sequence (same length as the original transposase). When transposase mRNA is present (either exogenous, when working in vitro, or endogenous, when in vivo) and an active transposase is being translated, transposition will occur, rendering EGFP+ cells. Note that transposition footprint is in frame with the egfp gene. The random sequence insertion sites can then be characterized using deep sequencing of reverse PCR amplicons. IR- inverted repeat. EGFP-enhanced green fluorescent protein. pA-polyadenylation signal. NNN-transposition footprint.