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. 2012 Jan 1;3(1):101–110. doi: 10.4161/nucl.18955

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Copyright © 2012 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name nucl-3-101-g3.jpg — Figure 3. Mapping of the M18BP1-CENP-C interaction domains by F3H. (A) Scheme of M18BP1 truncations used in the interaction tests. (B) Representative F3H images of the negative control (GFP only) and EGFP tagged M18BP1 truncations tested with RFP-CENP-C. Overlap of red and green signals at the nuclear lacO focus indicates interaction. (C) Quantification of the F3H M18BP1-CENP-C interaction data. Intensities of red and green signals at the nuclear lacO focus were measured in several hundred cells each. The ratio between red and green signals was determined to measure the strength of the tested interactions.