Figure 5. CENP-C knock-down leads to impaired centromeric recruitment of M18BP1. (A) RT-qPCR for CENP-C and M18BP1 five days after knock-down. Expression levels in the control knock-down cell line (shControl) and the three CENP-C knock-down cell lines (shCENP-C #1-#3) were normalized to the geometric mean of GAPDH and Actin. (B) Representative maximum intensity projections of confocal stacks of control and CENP-C knock-down cells that were stained for CENP-A. Arrows point to example cells for the three classes of M18BP1 signals: strong (s), weak (w) and no enrichment/diffuse nuclear (d). Scale bars are 10µm. (C) Quantification of the M18BP1 signals in control vs. CENP-C knock-down cell lines. M18BP1 and CENP-A staining patterns were classified in several hundred cells. The bar graph depicts the percentages of each class.