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Temporal effect of ASIL1 mutation on expression of seed maturation genes in developing seeds. Total RNA was isolated from siliques of wild type (Col-0) and asil1–1 corresponding to five developmental stages as indicated (DPA) and subjected to real-time RT-PCR analysis with ACT2 mRNA as an internal reference and gene-specific primers as previously described.1 The mean and sd from qRT-PCR analysis were determined from three biological replicates of either Col-0 or asil1–1.
