Table 2.
Experimental designs used in RSM studies by using four independent variables with six centre points showing observed and predicted values of protease production
| Standard Order |
Factor A (Glucose %, w/v) |
Factor B (Yeast extract %, w/v) |
Factor C (MgSO4 %, w/v ) | Factor D (pH) | Observed response (Uml-1) | Predicted response (Uml-1) |
| 1 | 0.75 | 0.30 | 0.0100 | 9.00 | 419 | 418.13 |
| 2 | 1.25 | 0.30 | 0.0100 | 9.00 | 457 | 455.63 |
| 3 | 0.75 | 0.70 | 0.0100 | 9.00 | 449 | 451.13 |
| 4 | 1.25 | 0.70 | 0.0100 | 9.00 | 482 | 483.63 |
| 5 | 1.00 | 0.50 | 0.0075 | 8.80 | 524 | 562.46 |
| 6 | 1.00 | 0.50 | 0.0125 | 8.80 | 540 | 541.96 |
| 7 | 1.00 | 0.50 | 0.0075 | 9.20 | 372 | 370.79 |
| 8 | 1.00 | 0.50 | 0.0125 | 9.20 | 396 | 394.29 |
| 9 | 0.75 | 0.50 | 0.0100 | 8.80 | 561 | 561.58 |
| 10 | 1.25 | 0.50 | 0.0100 | 8.80 | 617 | 612.58 |
| 11 | 0.75 | 0.50 | 0.0100 | 9.20 | 426 | 425.92 |
| 12 | 1.25 | 0.50 | 0.0100 | 9.20 | 447 | 444.92 |
| 13 | 1.00 | 0.30 | 0.0075 | 9.20 | 369 | 365.25 |
| 14 | 1.00 | 0.70 | 0.0075 | 9.00 | 415 | 413.75 |
| 15 | 1.00 | 0.30 | 0.0125 | 9.00 | 406 | 402.75 |
| 16 | 1.00 | 0.70 | 0.0125 | 9.00 | 416 | 415.25 |
| 17 | 0.75 | 0.50 | 0.0075 | 9.00 | 446 | 446.63 |
| 18 | 1.25 | 0.50 | 0.0075 | 9.00 | 479 | 482.13 |
| 19 | 0.75 | 0.50 | 0.0125 | 9.00 | 466 | 466.63 |
| 20 | 1.00 | 0.50 | 0.0125 | 9.00 | 498 | 501.13 |
| 21 | 1.00 | 0.30 | 0.0125 | 8.80 | 509 | 512.96 |
| 22 | 1.00 | 0.70 | 0.0100 | 8.80 | 513 | 511.46 |
| 23 | 1.00 | 0.30 | 0.0100 | 9.20 | 324 | 329.29 |
| 24 | 1.00 | 0.70 | 0.0100 | 9.20 | 392 | 391.79 |
| 25 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
| 26 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
| 27 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
| 28 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
| 29 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |
| 30 | 1.00 | 0.50 | 0.0100 | 9.00 | 514 | 514 |