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Diagram of GG-peptide enrichment strategy. (A) 8xHis-Ubiquitin is covalently attached to lysine residues of proteins. (B) Proteins are either mock treated or cleaved by addition of NTCB to the lysates. (C) Ubiquitinated proteins are purified by metal affinity resin. (D) Proteins are digested with trypsin. (E) Peptides are separated by strong cation exchange chromatography. (F) Peptides are identified by RPLC-MS/MS.
