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. Author manuscript; available in PMC: 2013 Apr 24.
Published in final edited form as: ACS Nano. 2012 Mar 14;6(4):3491–3498. doi: 10.1021/nn300524f

Figure 3. Cleavage assays of the MMP2-cleavable peptide and its conjugates.

Figure 3

The peptide and its conjugates were treated with the active human MMP2 at two concentrations, 1 and 10 ng/μL, in HBS at 37°C for 24h. The reactions were followed using both RP-HPLC and TLC. Panels A, B, D and E show the RP-HPLC chromatograms of the MMP2-cleavable peptide (A), MAL-PEG(3400)-peptide (B), MAL-PEG(3400)-peptide-DOPE (D), and the mixture of MAL-PEG(3400)-peptide and MAL-PEG(3400)-peptide-DOPE (E). Panels C and F show the TLC chromatograms of MAL-PEG(3400)-peptide (C) and MAL-PEG(3400)-peptide-DOPE (F). The conditions for HPLC and TLC were the same as those in the conjugate characterization section.